pdquest advanced 8 0 1 software  (Bio-Rad)

Journal: Journal of Veterinary Research

Article Title: Murine hepatic proteome adaptation to high-fat diets with different contents of saturated fatty acids and linoleic acid : α-linolenic acid polyunsaturated fatty acid ratios

doi: 10.2478/jvetres-2024-0041

Figure Lengend Snippet: Experimental design to elucidate liver proteomic profile in mice fed four fat-varied diets. PUFA – polyunsaturated fatty acids; STD – standard diet; SFA – diet high in saturated fatty acids; 14 :1 – diet high in PUFA with this linoleic acid to α-linolenic acid ratio; 5 : 1 – diet high in PUFA with this linoleic acid to α-linolenic acid ratio; qPCR – real-time quantitative polymerase chain reaction; 2DE – two-dimensional electrophoresis. PDQuest Advanced 8.0.1 (Bio-Rad Laboratories, Hercules, CA, USA) software used for analysis of protein profiles. MALDI-TOF/TOF – matrix-assisted laser desorption/ionisation–time-of-flight tandem mass spectrometry

Article Snippet: Sample preparation, isoelectrofocusing, sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), image staining and protein profiles analysis in the PDQuest Advanced 8.0.1 software (Bio-Rad Laboratories, Inc Hercules, CA, USA) were carried out as previously described ( ).

Techniques: Real-time Polymerase Chain Reaction, Electrophoresis, Software, Mass Spectrometry

Journal: International journal of molecular sciences

Article Title: Proteome-Level Investigation of Vitis amurensis Calli Transformed with a Constitutively Active, Ca 2+ -Independent Form of the Arabidopsis AtCPK1 Gene.

doi: 10.3390/ijms241713184

Figure Lengend Snippet: Figure 1. AtCPK1-Ca-triggered increased and decreased amounts of the proteins associated with signaling. Fragments of two-dimensional gels of protein fractions from the control (Va) and AtCPK1-Ca-transformed VaCa calli obtained in three separate experiments are presented in the left panel. Proteins were separated on gels by isoelectric mobility and by mass. Gels were scanned using a VersaDoc MP 4000 system with PDQuest 8.0.1 Advanced software, as described in Section 4.3. Protein expression is presented in Table 1 as the average total spot intensity in a group of three repeated gels. Mass spectra were acquired with a MALDI-TOF mass spectrometer and presented on the right panel. (a) bifunctional nitrilase/nitrilehydratase NIT4B; (b) calreticulin; (c) annexin; (d) protein EXORDIUM. Up-regulated proteins are marked with a green circle, down-regulated proteins are marked with a red circle, and newly arising proteins are marked with a blue circle.

Article Snippet: PDQuest 8.0.1 Advanced software (Bio-Rad Laboratories Inc., Hercules, CA, USA) was used for the analysis of the protein maps.

Techniques: Control, Transformation Assay, Software, Expressing, Mass Spectrometry

Journal: International journal of molecular sciences

Article Title: Proteome-Level Investigation of Vitis amurensis Calli Transformed with a Constitutively Active, Ca 2+ -Independent Form of the Arabidopsis AtCPK1 Gene.

doi: 10.3390/ijms241713184

Figure Lengend Snippet: Figure 3. AtCPK1-Ca-triggered increased and decreased amounts of the PR-proteins. Fragments of two-dimensional gels of protein fractions from the control (Va) and AtCPK1-Ca-transformed VaCa calli obtained in three separate experiments are presented in the left panel. Proteins were separated on gels by isoelectric mobility and by mass. Gels were scanned using a VersaDoc MP 4000 system with PDQuest 8.0.1 Advanced software, as described in Section 4.3. Protein expression is presented in Table 1 as the average total spot intensity in a group of three repeated gels. Mass spectra were acquired with a MALDI-TOF mass spectrometer and presented on the right panel. (a) betvI/major latex protein; (b) osmotin-like protein 13; (c) thaumatin-like protein; (d) beta-D-xylosidase 1; (e) class IV chitinase. Up-regulated proteins are marked with a green circle, down-regulated proteins are marked with a red circle, and newly arising proteins are marked with a blue circle.

Article Snippet: PDQuest 8.0.1 Advanced software (Bio-Rad Laboratories Inc., Hercules, CA, USA) was used for the analysis of the protein maps.

Techniques: Control, Transformation Assay, Software, Expressing, Mass Spectrometry

Journal: International journal of molecular sciences

Article Title: Proteome-Level Investigation of Vitis amurensis Calli Transformed with a Constitutively Active, Ca 2+ -Independent Form of the Arabidopsis AtCPK1 Gene.

doi: 10.3390/ijms241713184

Figure Lengend Snippet: Figure 4. AtCPK1-Ca-triggered increased and decreased amounts of the HSP proteins. Fragments of two-dimensional gels of protein fractions from the control (Va) and AtCPK1-Ca-transformed VaCa calli obtained in three separate experiments are presented in the left panel. Protein was separated on gels by isoelectric mobility and by mass. Gels were scanned using a VersaDoc MP 4000 system with PDQuest 8.0.1 Advanced software, as described in Section 4.3. Protein expression is presented in Table 1 as the average total spot intensity in a group of three repeated gels. Mass spectra were acquired with a MALDI-TOF mass spectrometer and presented on the right panel. (a) heat shock cognate 70 kDa protein; (b) histidine kinase/HSP90-like ATPase domain-containing protein. Up-regulated proteins are marked with a green circle, down-regulated proteins are marked with a red circle, and newly arising proteins are marked with a blue circle.

Article Snippet: PDQuest 8.0.1 Advanced software (Bio-Rad Laboratories Inc., Hercules, CA, USA) was used for the analysis of the protein maps.

Techniques: Control, Transformation Assay, Software, Expressing, Mass Spectrometry

Journal: PLoS ONE

Article Title: Proteomic Analysis of the Low Mutation Rate of Diploid Male Gametes Induced by Colchicine in Ginkgo biloba L.

doi: 10.1371/journal.pone.0076088

Figure Lengend Snippet: Obs. pI and Mr, observed pI, Mr calculated from the 2D-gels with PDQuest 8.0.1 software according to standard marker proteins.

Article Snippet: The image analysis was carried out by a combination of manual visualization and software calculations using PDQuest 8.0.1 Gel Analysis Software (Bio-Rad, Hercules, CA, USA).

Techniques: Software, Marker, Transduction, Sequencing